DL-PSI-486 Single-channel Pilot-type Automatic Peptide Synthesizer
PSI486 single-channel pilot-type fully automatic peptide synthesis instrument is a standing solid-phase peptide synthesis instrument for pilot-scale production of peptides.Fully automated greatly saves labour cost,only need to set the order of amino acid addition,time duration,etc.in the software,and then the synthesis can be started automatically according to the program. Nitrogen/solvent is alternated periodically to clean the pipeline automatically.
I. Working Principle
The production automatic peptide synthesizer combines solid-phase synthesis technology with automated control technology. Its core steps are as follows:
Solid Phase Carrier Selection: Select a suitable solid phase carrier to attach the first amino acid to the resin through covalent bonding.
Amino acid activation: activate the carboxyl group of the amino acid using specific chemical reactions to make it easier to react with the amino group on the resin.
Coupling One by One: Amino acids are coupled to the growing polypeptide chain one by one through an automated system. After each coupling step, a washing and deprotection operation is performed to ensure that the next amino acid will be properly attached.
Cleavage and purification: When all amino acids are coupled, the peptide is released from the resin by specific cleavage conditions and subsequent purification and separation operations are performed.
Production Automatic Peptide Synthesizer
II. Advantages
High efficiency: It can significantly improve the efficiency of peptide synthesis and shorten the production cycle. Compared with the traditional manual synthesis method, the automated system can complete the synthesis of a large number of peptides in a short period of time.
Accuracy: The automated system can precisely control the conditions and parameters of each step of the reaction, ensuring that the synthesized peptides have a high degree of accuracy and consistency.
Flexibility: The synthesis scheme can be flexibly adjusted according to the needs, including amino acid sequence, length, etc., to meet the needs of different application scenarios.
Scalability: The Production Automatic Peptide Synthesizer can realize large-scale production, reduce production costs and improve market competitiveness.
Safety: The automated system reduces the number of manual operation steps, which reduces the possibility of operation errors and safety risks.
With its many advantages of high efficiency, precision, flexibility, scalability and safety, the Production Automatic Peptide Synthesizer shows great potential for application in the field of peptide production.
III. Operation process of six-channel peptide synthesizer:
1. Prepare raw materials: choose suitable amino acid resins, protective groups and condensation reagents. Make sure all reagents and solvents are dry to avoid hydrolysis reaction.
2. Load resin: Load amino acid resin into the reaction column of the synthesizer. The resin can be evenly distributed in the six channels to ensure the synthesis efficiency and quality of each peptide chain.
3. Amino acid coupling: Mix the desired amino acids with appropriate condensation reagents and add them to the reaction column. The coupling reaction usually takes some time to ensure that the amino acids are completely bound to the resin.
4. Removal of Protective Groups: After the coupling of all amino acids is complete, the protective groups need to be removed in order to expose the amino groups in preparation for the next round of coupling.
5. Cleaning and de-activation: After deprotection, the resin needs to be thoroughly cleaned and residual reactive groups need to be de-activated to prevent them from interfering with subsequent reactions.
6. Successive cycles: Repeat the above steps until the target peptide is synthesized. Each cycle needs to ensure the complete coupling of amino acids and the complete removal of protective groups.
Six-channel Peptide Synthesizer
IIII.Technical points:
1. Selection of solid-phase carrier: Selection of a suitable solid-phase carrier (e.g., resin) is crucial for peptide synthesis. The type and nature of the resin will affect the speed and efficiency of the synthesis.
2. Condensation reaction: Condensation reaction is a key step in peptide synthesis, and efficient condensation reagents need to be selected to ensure that the bonding between amino acids is complete and reversible.
3. Protection strategies: In peptide synthesis, the side chains of amino acids usually need to be protected to prevent them from reacting unnecessarily during the condensation process. Choosing the right protecting group and controlling the conditions for its deprotection is the key to the success of the synthesis.
4. Waste disposal: Waste and unreacted reagents generated during the synthesis process need to be properly disposed of to minimize environmental pollution and ensure laboratory safety.
5. Quality control: Throughout the synthesis process, regular quality control tests are required to ensure that each step of the reaction is carried out as planned and that the synthesized peptide meets the predetermined specifications and purity requirements.
The operation of the six-channel peptide synthesizer requires fine chemical reaction control and strict process management. A good understanding of the synthesizer's operating procedures and technical points is essential to improve the efficiency and quality of peptide synthesis.
It has wide application prospects in automated peptide chain synthesis. It can be used to synthesize peptide compounds with specific functions, such as antibodies, enzyme inhibitors, hormones and so on. In addition, the single-channel peptide synthesizer can also be used to study the relationship between peptide chain structure and function, providing an important basis for drug design.
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